pathscan total notch1 sandwich elisa kit  (Cell Signaling Technology Inc)

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Fully human monoclonal antibody targeting activated ADAM10 on colorectal cancer cells

doi: 10.1016/j.biopha.2023.114494

Figure Lengend Snippet: 1H5 inhibits Notch cleavage. Sandwich ELISA was used to measure the levels of total (A) and cleaved (B) Notch1 in COLO205 cells upon treatment with 1H5. The data represent mean of triplicate experiments, and the bar plots show the effect of treatment with 1H5 relative to untreated control, mean ± SEM. Comparison of notch levels between treated and untreated groups was performed using independent t test. Total Notch1 levels did not significantly differ between the two groups (A), p = 0.162. On the other hand, the mAb-treated group showed significant decrease of the cleaved Notch1 levels when compared with the untreated control (B), p < 0.001.

Article Snippet: We used the PathScan ® total Notch1 sandwich ELISA kit to measure total Notch1 according to the manufacturer’s protocol (Cell Signaling Technologies).

Techniques: Sandwich ELISA, Control, Comparison

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Fully human monoclonal antibody targeting activated ADAM10 on colorectal cancer cells

doi: 10.1016/j.biopha.2023.114494

Figure Lengend Snippet: 1H5 inhibits Notch cleavage. Sandwich ELISA was used to measure the levels of total (A) and cleaved (B) Notch1 in COLO205 cells upon treatment with 1H5. The data represent mean of triplicate experiments, and the bar plots show the effect of treatment with 1H5 relative to untreated control, mean ± SEM. Comparison of notch levels between treated and untreated groups was performed using independent t test. Total Notch1 levels did not significantly differ between the two groups (A), p = 0.162. On the other hand, the mAb-treated group showed significant decrease of the cleaved Notch1 levels when compared with the untreated control (B), p < 0.001.

Article Snippet: We used the PathScan ® total Notch1 sandwich ELISA kit to measure total Notch1 according to the manufacturer’s protocol (Cell Signaling Technologies).

Techniques: Sandwich ELISA, Comparison

Journal: Arthritis research & therapy

Article Title: Notch signaling is activated in knee-innervating dorsal root ganglia in experimental models of osteoarthritis joint pain.

doi: 10.1186/s13075-023-03039-1

Figure Lengend Snippet: Fig. 3 Intra-articular injection of LPS activates Notch signaling in knee-innervating DRG of naïve mice. A Knee hyperalgesia 24-h time-course after IA injection of LPS (3 μg) or vehicle in 10-week old naïve mice (N = 6 mice per group). 450 g is the threshold baseline for naïve mice (dashed line). B Gene expression of Adam17, Rbpj, Hes1, Jag1, and Notch1 in the ipsilateral L3-L5 DRGs 4 h after IA injection of LPS or vehicle (N = 6 mice per group). C Protein levels of NICD in the pooled L3-L5 DRG tissue lysates 24 h after IA injection of LPS or vehicle (N = 4 mice per group). NICD levels are presented as absorbance value at 450 nm normalized to total protein (A450/mg total protein). D Gene expression of Ccl2 in DRG 4 h after IA injection of LPS or vehicle (N = 6 mice per group). E Protein levels of CCL2 in DRG tissue lysates 24 h after IA injection of LPS or vehicle (N = 4 mice per group). CCL2 levels are presented as CCL2 protein normalized to total protein (pg CCL2/mg total protein)

Article Snippet: Protein levels of NICD, the active form of Notch protein, in L3-L5 DRG from mice 12 or 26 weeks after DMM or sham surgery (N = 4–7 mice per group) were assessed using PathScan Cleaved Notch1 Sandwich ELISA Kit (Cell Signaling Technology, Danvers, MA) following the manufacturer’s instructions.

Techniques: Injection, Gene Expression

Journal: Arthritis research & therapy

Article Title: Notch signaling is activated in knee-innervating dorsal root ganglia in experimental models of osteoarthritis joint pain.

doi: 10.1186/s13075-023-03039-1

Figure Lengend Snippet: Fig. 6 Notch signaling genes are expressed in DRG neurons after DMM surgery. RNA in situ hybridization analysis of Jag1 (A), Hes1 (B), and Notch1 (C) in the L4-DRG of mice 26 weeks after DMM or sham surgery. Representative images of each group are shown (N = 3 mice per group). Scale bars, 25 μm. RNA expression in the neurons was quantified using H-score

Article Snippet: Protein levels of NICD, the active form of Notch protein, in L3-L5 DRG from mice 12 or 26 weeks after DMM or sham surgery (N = 4–7 mice per group) were assessed using PathScan Cleaved Notch1 Sandwich ELISA Kit (Cell Signaling Technology, Danvers, MA) following the manufacturer’s instructions.

Techniques: RNA In Situ Hybridization, RNA Expression

Journal: Journal of proteome research

Article Title: Tbx18 Orchestrates Cytostructural Transdifferentiation of Cardiomyocytes to Pacemaker Cells by Recruiting the Epithelial-Mesenchymal Transition Program.

doi: 10.1021/acs.jproteome.2c00133

Figure Lengend Snippet: Figure 5. Hallmarks of NRVM/PM transdifferentiation: electrogenic remodeling and EMT biosignature. (A) Differentially regulated (p < 0.05) ion channels. (B) Differentially regulated active transporters and exchangers. (C) Gap junctions in the Tbx18-iPMs. (D) Interferon-induced proteins. (E) Growth factor Signaling. (F) Heat map of EMT-associated proteins found in MSigDB (hierarchically clustered by sample, proteins ranked by inter- group variance). (G) Immunoblot analysis of key regulators of the EMT, Notch1, and Snai1.

Article Snippet: Transferred PVDF membranes were incubated with primary antibodies against total Notch1 (Cell Signaling Technology #3608, 1:1000) and Snail (Invitrogen #MA5-14801, 1:1000) overnight at 4 °C.

Techniques: Western Blot

Journal: iScience

Article Title: Heme oxygenase-1 mitigates liver injury and fibrosis via modulation of LNX1/Notch1 pathway in myeloid cells

doi: 10.1016/j.isci.2022.104983

Figure Lengend Snippet: A crosstalk of HO-1 and Notch1 signaling in Mφ (A and B) Immunostaining with antibody against Notch1 total was performed in the livers isolated from Hmox1 flfl or LysM-Cre:Hmox1 flfl ( Cre:Hmox1 flfl ) mice after BDL surgery. Scale bar: 100 μm (insets: 400x magnification). The quantification of a number of Notch1+ cells is shown in (B). Data are represented as mean ± SEM. ANOVA, p < 0.001, Tukey’s multiple comparison test: ∗p < 0.05, ∗∗∗p < 0.001. (C–E) LysM-Cre:RiboTag ( Hmox1 flfl ) or LysMCre:Hmox1 flfl : RiboTag ( Cre:Hmox1 flfl ) mice were subjected to BDL surgery as in Figure 2 . RT-PCR was performed using RNA isolated from the immunoprecipitates with the anti-HA antibody. Data are represented as mean ± SEM. ANOVA, p < 0.0001, Tukey’s multiple comparison test: ∗∗∗p < 0.001, ∗∗p < 0.01. n = 5–18 female and male mice per group. (F) Scheme representing the interaction of LNX1 in HO-1 and regulation of Mφ phenotype during liver injury.

Article Snippet: Anti-Total Notch1 , Novus Biologicals , Cat#: NBP-78292.

Techniques: Immunostaining, Isolation, Comparison, Reverse Transcription Polymerase Chain Reaction

Journal: iScience

Article Title: Heme oxygenase-1 mitigates liver injury and fibrosis via modulation of LNX1/Notch1 pathway in myeloid cells

doi: 10.1016/j.isci.2022.104983

Figure Lengend Snippet:

Article Snippet: Anti-Total Notch1 , Novus Biologicals , Cat#: NBP-78292.

Techniques: Recombinant, Protease Inhibitor, RNA Sequencing, Expressing, Plasmid Preparation, Software, Gene Expression